rat adiponectin elisa kit  (Millipore)

Journal: Plants

Article Title: Gut–Liver Axis-Mediated Anti-Obesity Effects and Viscosity Characterization of a Homogenized Viscous Vegetable Mixture in Mice Fed a High-Fat Diet

doi: 10.3390/plants14162510

Figure Lengend Snippet: Effects of the homogenized viscous vegetable mixture on lipid metabolism in high-fat (HF) diet fed mice. ( A ) Serum triglyceride (TG), ( B ) serum total cholesterol (TC), ( C ) serum low-density lipoprotein cholesterol (LDL-C), ( D ) serum high-density lipoprotein cholesterol (HDL-C), ( E ) LDL-C/HDL-C ratio, ( F ) liver TG, ( G ) liver TC, ( H ) plasma adiponectin, ( I ) plasma leptin, ( J ) adiponectin/leptin ratio. Data are expressed as the mean ± standard deviation (SD) ( n = 7). Significance between two groups was determined using Student’s t -test. p < 0.05. * represents a significant difference with the C group, # represents a significant difference with the H group; + represents a significant difference with the CV group.

Article Snippet: Plasma adipokines levels were detected with a Leptin Mouse/Rat ELISA kit (Biovendor, Brno, Czech Republic) and a Rat Adiponectin ELISA kit (Assaypro, Charles, MO, USA), following the manufacturer’s instructions.

Techniques: Clinical Proteomics, Standard Deviation

Journal: Plants

Article Title: Gut–Liver Axis-Mediated Anti-Obesity Effects and Viscosity Characterization of a Homogenized Viscous Vegetable Mixture in Mice Fed a High-Fat Diet

doi: 10.3390/plants14162510

Figure Lengend Snippet: Effects of the homogenized viscous vegetable mixture on hepatic mRNA expressions related to fatty acid synthesis ( A – E ), fatty acid oxidation ( F – M ) and cholesterol-metabolism ( N , O ) in high-fat (HF) diet-fed mice. Data are expressed as the mean ± standard deviation (SD) ( n = 7). Significance between two groups was determined using Student’s t -test. p < 0.05. * represents a significant difference with the C group, # represents a significant difference with the H group; + represents a significant difference with the CV group. SREBP, sterol regulatory element-binding protein; AMPKα, adenosine monophosphate-activated protein kinaseα; ACC, acetyl-CoA carboxylase; FAS, fatty acid synthase; SCD1, stearoyl-CoA desaturase 1; PPAR, peroxisome proliferator-activated receptor; PGC1, peroxisome proliferator-activated receptor gamma coactivator 1; SIRT1, sirtuin 1; AdipoR2, adiponectin receptor 2; MCAD, medium-chain acyl-CoA dehydrogenase; ACO1, acyl-CoA oxidase 1; CPT1, carnitine palmitoyltransferase 1; CYP7A1, cholesterol 7 alpha-hydroxylase.

Article Snippet: Plasma adipokines levels were detected with a Leptin Mouse/Rat ELISA kit (Biovendor, Brno, Czech Republic) and a Rat Adiponectin ELISA kit (Assaypro, Charles, MO, USA), following the manufacturer’s instructions.

Techniques: Standard Deviation, Binding Assay

Journal: ACS Omega

Article Title: Oral Administration of the Adiponectin Receptor 1 Agonistic Dipeptide Tyr-Pro Prevents Hyperglycemia in Spontaneously Diabetic Torii Rats

doi: 10.1021/acsomega.4c09030

Figure Lengend Snippet: Involvement of Tyr-Pro in glucose uptake-related signaling pathways, AdipoR1-mediated Signaling Pathway, and insulin signaling pathway in SDT Rats. Plasma insulin (A) and adiponectin (B) levels in SDT rats euthanized at 29 weeks of age were measured using ELISA insulin/adiponectin assay kits. The protein expression of (C) AdipoR1, (D) p -AMPK/AMPK, (E) GLUT4, and (F) p -IRS-1(Tyr608)/IRS-1 and p -IRS-1(Ser612)/IRS-1 in skeletal muscle tissue of SDT rats euthanized at 29 weeks of age was measured using Wes analysis. Protein expression was normalized by the electropherogram peak area of total protein in each lane. The chemiluminescent signal was displayed as a virtual blot-like image, and an electropherogram was generated based on the molecular weight. Results are presented as the mean ± SEM ( n = 3–4). Statistical analyses between the two groups were evaluated by Student’s t -test at p < 0.05, p < 0.01, p < 0.001, and N . S .: not significant at p < 0.05.

Article Snippet: Plasma adiponectin levels were determined using a rat adiponectin ELISA kit (HMW adiponectin, Lot: 634–13071, FUJIFILM Wako Shibayagi Co.) according to the manufacturer’s instructions.

Techniques: Protein-Protein interactions, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Expressing, Generated, Molecular Weight